GENERAL INFORMATION

Name : KEITH DWAIN CRAWFORD

Education :

1982 B.S. (Biology/Chemistry), Summa cum laude, PRAIRIE VIEW A&M UNIVERSITY
1986 M.D. (Medicine), UNIVERSITY OF TEXAS MEDICAL SCHOOL
1997 PH.D. (Immunology), HARVARD UNIVERSITY, Cambridge,MA

Postdoctoral Training :

01/86-12/87 - Intern in Surgery , Surgery, Howard University Hospital, Washington, D.C.
01/87-12/88 - Surgical Resident, Surgery, Howard University Hospital, Washington D.C.
01/88-12/91 - Predoctoral Research , Center for Blood Research
01/91-12/93 - NIH Predoctoral Research Fellow, Center for Blood Research

Academic Appointments :

1997-2004 - Research Fellow in Pediatrics, Pediatrics-Children's Hospital, Boston, MA
2004 -        - Instructor Harvard Medical School; Department of Orthopaedic Surgery,                      Brigham and Women's Hospital, Boston, MA

Hospital or Affiliated Institution Appointments :

1998 - Visiting Professor , Prairie View A&M University, Prairie View, TX


Hospital and Health Care Organization Clinical Service Responsibilities :

2004 - Committe for Clinical Investigation; Department of Orthopedics, Brigham and Women's Hospita


Major Committee Assignments:

2002 - BBRI Corporator, Associate Broad Member, Boston Biomedical Research Institute
2005 - Clinical Investigation Committee, Member, Brigham and Women's Hospital


Professional Societies:

1986 - American Medical Association Member, Member
1988 - National Medical Association, Member
2004 - American Society of Human Genetics , Member
2004 - American Association of Immunologist , Member


Community Service Related to Professional Work:

2005 - Katrina Relief Coordinator, The City of Newton and Myrtle Baptist Church
2005 - Mississippi Angel Adoption Coordinator , City of Newton and Myrtle Baptist Church

Awards and Honors:

1988 -            Outstanding Surgical Resident, Meharry Medical College
1997 -            Harvard AIDS Fellowship, Dana-Faber Cancer Institute/The Center for Blood                        Research
1999 -             Department of Defense Sponsored Research Consortium, Center for Blood                        Research
1999 - 2003 - Robert Wood Johnson Foundation Harold Amos Scholar, The Center for Blood                        Research


Part II: Research, Teaching, and Clinical Contributions

A. Narrative report of Research, Teaching, and Clinical Contributions

I. Determine the role of dendritic cells in the host's response to various pathogens and develop a sensitive and high-throughput method for the detection acute exposure to biothreats. Preliminary findings suggest that dendritic cells and monocytes, when exposed to chemical agents or Bacillus anthracis, reveal pathogen-specific gene and protein profiles. These findings support the use of gene and protein expression patterns as a mean of detecting exposure pathogens in asymptomatic patients.

II. Investigate the role of the immune cells in adult stem cell proliferation and differentiation. Preliminary studies demonstrate that human dendritic cells not only are responsible for the initiation of innate and adaptive immune responses, but are also involved in adult stem cell proliferation. These finding support the possible use of dendritic cells to augment adult stem expansion.

B. Funding Information

1998-1999 - Post Doctoral , N.I.H. , Role of dendritic cells in HIV-1 pathogenesis
1999-2002 - Co-P.I., D.O.D., Development of antidotes that protect the public after                      chemical and biological weapon exposure
1999-2003 - Fellow, Robert Wood Johnson Foundation, Role of primary blood dendritic cells                      in innate and adaptive immunity
2003-2005 - Co-P.I., D.O.D., DAMD17-03-0054, Construction of a normalized cDNA library                      designed to allow the detection of redox-related gene expression in various                      tissues exposed to CEES and other cytopathic compounds
2003-2007 - Investigator, N.I.H., 5R01AR050243-02 , Healing of segmental defects in bone                      by gene transfer
2007-2008 - Project Director, D.O.D., DAMD17-03-0054, Development of the APC-                      biosensor and host response reference library

C. Report of Other (Non-Funded) Activities

Board member Southeast Texas Biomedical Research Institute

D. Report of Teaching

1. Local contributions

c. Local Invited Presentations

Grand Rounds

2007 - The Future of Stem Cells in Orthopedics, Brigham and Women's Hospital

Seminar

2005 - Immunology of CD2+ Dendritic Cells: Another Potential Source of Potent APCs in
           Cancer Immunotherapy, Dana Farber Cancer Institute
2006 - Future Opportunties in Biomedical Research, University of Massachusetts Boston
2007 - New Techiques in Q-PCR and High Through Automation, Harvard Medical School

e. Advisory and Supervisory Responsibilities in Clinical or Laboratory Setting

1997 - 200 High School, Undergraduate, and Graduate Students for 200 hrs/year, Mentoring             in Biomedical Science Careers Program, Harvard University, Prairie View A&M             University, Boston & Newton Public  Schools

f. Leadership Roles

2002 - Fenway Intern Supervisor, Brigham and Women's Hospital
Responsibility : Designing and Implementing Molecular Biological Experiments for Fenway High School Students

2. Regional, national, or international contributions :

a. Invited Presentations
Regional

2005 - APC-biosensor: use of dendritic cells and monocytes for early detection of exposure             to toxic substances, Prairie View A&M University[Invited Lecture]
2006 - APC-biosensor: use of dendritic cells and monocytes for the early detection of             exposure to toxic substances, US Congress[Invited Lecture]

National

2005 - APC-biosensor: use of dendritic cells and monocytes for early detection of exposure             to toxic substances, In-vitro Biology [Invited Lecture]
2006 - APC-biosensor: use of dendritic cells and monocytes for the early detection of             exposure to toxic substances, Department of Defense [Invited Lecture]
2007 - Purpose of the presentation was to educated those individuals, communities, political             leaders, and public policy makers who were interested in knowing more about stem             cells and how stem cell therapies will impact the future of healthcare, National             Conference on Health Disparities [Invited Lecture]
2007 - Anthrax and Yersina Pestis-Specific Host Transcriptional Response, Health &
           Human Services: Biomedical Advance Research and Development Authority (BARDA)
           [Invited Lecture]

Part III: Bibliography
Original Articles

1.Gabuzda DH, Li H, Lawrence K, Vasir B, Crawford K, Langhoff E. Essential role of vif in establishing productive HIV-1 Infection in peripheral blood T lymphocytes and monocyte/macrophages. J Acquir Immune Defic Syndr. 1994;7:908-15.

2.Crawford K, Gabuzda D, Xu J, Pantozopoulos V, Clement C, Reinherz E, Alper CA.Circulating CD2+
monocytes are dendritic cells. J Immunol. 1999;163:5920-8.

3.Crawford K, Alper CA. Genetics of the complement system. Rev Immunogenet. 2000;2:323-38.

4.Gorry PR, Bristol G, Zack JA, Ritola K, Swanstrom R, Birch CJ, Bell JE, Norbert N, Crawford K, Wang H, Schols D, De Clercq E, Kunstman K, Wolinsky SM, Gabuzda D. Macrophage tropism of human immunodeficiency virus type 1 isolates from brain and lymphoid tissues predicts neurotropism independent of coreceptor specificity. J Virol. 2001: pp. 10073-89.

5.Uko GP, Fraser PA, Awdeh ZL, Fici DA, Crawford KD, Larsen CE, Alper CA. Hepatitis B surface antigen- and tetanus toxoid-specific clonal expansion of CD4+ cells in vitro determined by TCRBV CDR3 length and nucleotide sequence. Genes Immun. 2001;2:11-19.

6.Wang J, Crawford K, Yuan M, Wang H, Gorry PR, Gabuzda D. Regulation of CC chemokine receptor 5 and CD4 expression and human immunodeficiency virus type 1 replication in human macrophages and microglia by T helper type 2 cytokines. J Infect Dis . 2002;185:885-97.

7.Crawford K, Stark A, Kitchens B, Sternheim K, Pantazopoulos V, Triantafellow E, Wang Z, Vasir B, Larsen CE, Gabuzda D, Reinherz E, Alper CA. CD2 engagement induces dendritic cell activation: implications for immune surveillance and T-cell activation. Blood. 2003;102:1745-52.

8.Song E, Lee SK, Dykxhoorn DM, Novina C, Zhang D, Crawford K, Cerny J, Sharp PA, Lieberman J, Manjunath N, Shankar P. Sustained small interfering RNA-mediated human immunodeficiency virus type 1 inhibition in primary macrophages. J Virol. 2003;77(13):7174-81.

9.Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D. Dendritic Cells Induce MUC1 Expression and Polarization on Human T Cells by an IL-7-Dependent Mechanism. J Immunol. 2005;174:2376-86.

Proceedings of Meetings

1.Crawford K, Alper, C Tedder T. Circulating human blood dendritic cells express the HB-15 surface antigen (CD83), which is not detected on other blood leukocytes. In: Proceedings 34th Annual Meeting American Society of Hematology; Dec 4-8; Anaheim, CA. ;1992. p. .

2.Crawford K, Alper C, Shi B, Vasir B, Gabuzda D. A subpopulation of immature blood dendritic cells is highly susceptible to infection by macrophage-tropic HIV-1. In: Proceedings 4th Conference on Retroviruses and Opportunistic Infectionsroceedings; January 22-26; Washington DC. ;1997. p. .

3.Crawford K, Alper C, Shi B, Vasir B, Gabuzda D. A subpopulation of immature blood dendritic cells is highly susceptible to infection by macrophage-tropic HIV-1. In: Proceedings 9th Meeting of the National Cooperative Vaccine Development Groups for AIDS; May 4-7; NIH, Bethesda, MD. ;1997. p. .

4.Crawford K. CEES induces cellular apoptosis via the caspase pathways: implications for the development of effective countermeasures. In: Proceedings of the Mustard Consortium; October 15; Alexandria, Va. ;2000. p. .

5.Turnquist S, Crawford KD. N-acetylcysteine prevents CEES induced apoptosis: implications for possible antidote for chemical and biological weapons. In: Proceedings of Chemical Weapons Defense Institute, Aberdeen Providing Grounds; ,October 18; Aberdeen, Maryland. ;2002. p. .

6.Crawford KD, Wang C, Kitchens B, Pantazopoulos V, Xu J, Gabuzda D, Reinherz E, Alper CA. CD2 engagement induces dendritic cell activation: implications for immune surveillance and T cell activation. In: May 21; Boston, MA,. ;2003. p. .

7.Turnquist, S., Smith, M., Crawford, K. The Effect of Sulfur-mustard on Primary Macrophages. In: USAMRICD. Poster Presentation; May 18-20, 2004 ; Hunt Valley, Maryland. ;2004. p. .

Reviews/Chapters/Editorials

1. Crawford K, Alper CA. Genetics of the complement system. Rev Immunogenetics. 2000;2 :323-38.

2.Smith MG, Stone W, Crawford K, Ward P, Till G, Das S. Are Antioxidant Liposomes (STIMAL) The Universal Antidote to Several Weapons of Mass Destruction? Jane’s Online. 2003.

Thesis :

1.Crawford KD. Human peripheral blood dendritic cells: phenotype, morphology, and function. Boston (MA): Harvard University;1997.

Patents :

1.Crawford KD Alper CA; Center for Blood Research. Enrichment of dendritic cells from blood.
US 6,194,204 B1. 2001 Feb 27.

2.Crawford, KD Alper CA; Center for Blood Research. Enrichment of dendritic cells from blood.
US 6,589,526 B2. 2003 Jun 8.

3.Crawford, KD Alper CA; Center for Blood Research. Selective activation of dendritic cell-based
vaccines. pending. 2004 Jan 1.

4.Crawford K. Immune Cell Biosensors and Methods using same. pending. 2004 Sep 2.

5.Keith D. Crawford; Partners Healthcare. Stem Cells and Isolation Thereof. 2007 May 18.

Abstracts :

1. Alper C, Clement C, Gabuzda D, Reinherz E, Crawford K. CD2 distinguishes human blood dendritic cell precursors from monocytes. FASEB J. 1998;12:A275.

2.Crawford K, Wang C, Kitchens B, Gabuzda D, Reinherz E, Alper A. CD2-induced activation of human blood dendritic cell precursors. FASEB J. 1998;12:A300.

3.Crawford K, Wang C, Kitchens B, Pantazopoulos V, Xu J, Gabuzda D, Reinherz E, Alper CA. CD2 ligation induces blood dendritic cell activation. FOCUS. 2003:1, 3.

4.Keith D. Crawford, Shawn Turnquist, Baldev Vasir, Rudolf Fluckiger, Katrine Cosmopolous, and Chester Alper . Dendritic Cells and Monocytes Differentially Secret Complement Protein C3 in Response to Various Stimuli . 2005.

5.Crawford KD, Joo JJ, Fluckiger R, Vasir D, de Arce V, Evans CH, Thornhill TS . Identification of dendritic cells and CD133+ stem cells in the synovial fluid of osteoarthritic patients . Orhtopedic Research Society. 2007;Accepted.